Quantitative evaluation of the effects of a finished product or active ingredient on cell proliferation and protein synthesis through MTT test and total protein quantification after different periods of exposure.
Hair products and cosmetics products for scalp treatment.
Description of the test:
In vivo test: performed on selected volunteers with specific trichological features (dry and damaged hair). Volunteers will apply the product according to pre-agreed frequency and methods. 10 hairs from each subject will be gathered at T=0 and T= X days (duration to be defined with the customer). Dynamometric measurements will be performed in order to evaluate the increase in tensile strength of the hairs and their resistance to breakage. Subjective evaluations will be provided by volunteers using an open and multiple choices questionnaire to verify the perceived efficacy and other parameters such as effects on hair volume and agreeability. In vitro test: standardized locks of human hair (several types are available according to preferences) will be treated with a damaging mixture in controlled conditions. Part of the locks will then undergo treatment with the product. Comparative imaging will be performed under tangent light to highlight improvement of hair brightness. 2 samples will be taken from each lock to evaluate improvement by phase contrast microscopy or SEM.
Clinical objective evaluation with high resolution imaging. Pull test: count of the number of hairs collected under standardized traction. Trichographic analysis through cell biology techniques, and evaluation of the biological activity of collected hairs from each volunteer through MTT evaluation of cell vitality.
Hair density in the target region (number of hairs/cm2);
Dynamometric analysis of the resistance to combing on standardized hair locks. Comparison between treated and untreated wet locks. Results in terms of combing friction (in Newtons) and percentage of friction reduction compared to the untreated control.
Dynamometric analysis of the tensile strength and resistance to breakage on treated and untreated hairs collected ex vivo from 20 volunteer. Results in terms of tensile strength (in Newtons) and percentage of strengthening compared to the untreated control.
Quantification of the changes in the gene expression of 5α-reductase in murine Balb/3T3 fibroblasts. Demonstration of the efficacy of the ingredient or finished product in reducing the expression of mRNA codifying for 5α-reductase type I or II.
Analysis of the in vitro expression of the 5α-reductase enzyme by real time PCR in comparison with untreated substrates for the evaluation of inhibition effects. Demonstration of the efficacy of the ingredient or finished product in reducing the expression of mRNA codifying for 5α-reductase type I or II.
The ACCREDIA Certification, issued by the Italian Accreditation body ACCREDIA, certifies that the Abich Laboratory complies with the requirements of the UNI CEI EN ISO / IEC 17025: 2018 standard for tests on surgical masks. Accredited tests are Bacterial Filtration Efficiency (BFE) and Microbial Cleanliness (Bioburden).