Regulatory reference: | According to OECD 456. |
Claim: | No endocrine disruption |
Application field: | Raw ingredients and finished products. |
Description of the test: | The purpose of the test is identifying substances that interfere with 17-ß estradiol and testosterone production. |
Regulatory reference: | ISO 24444:2010 of 15/11/2010 and Colipa test method 2006 for the determination of the WATER RESISTANCE. Test performed according to modified COLIPA 2005 method |
Claim: | Salt water resistant |
Application field: | Finished sunscreen products. |
Description of the test: | Test performed on 10 volunteers, by comparison of pre-wash and post-wash SPF. The treatment includes two washes, one in salt water, performed by irroration with 1M NaCl solution by a pressureless drop shower, and one in fresh water. If the reduction of protection is greater than 50% the product isn’t considered to be “Salt Water Resistant”. |
Regulatory reference: | Proprietary method. |
Claim: | Anti-aging and Reduces signs of aging |
Application field: | Raw materials and finished products. |
Description of the test: | In vitro evaluation of the synthesis of hyaluronic acid up to 48h following exposure to the sample, in several concentrations, on skin and dermal papillae fibroblasts. |
Regulatory reference: | ISO 24444:2010 of 15/11/2010 and Colipa test method 2006 for SPF. |
Claim: | Sand resistant |
Application field: | Finished products. |
Description of the test: | In vivo test on 10 subjects. Comparison between SPF of sun product and the reduction on protection cause of double sand static contact of 20 minutes in this sequence: contact, techanical cleaning, contact and final mechanical cleaning before the exposure to the solar multiport simulator. If the reduction of protection is mayor than 50% the product isn’t “sand resistant”. |
Regulatory reference: | Proprietary method |
Claim: | Anti-pollution and Antioxidant |
Application field: | Raw materials and finished products. |
Description of the test: | In vitro test for the evaluation of the protective effect against air pollution. The purpose of this test is to evaluate the efficacy of the sample in reducing ROS release in response to a mix of heavy metals and atmospheric dust particulate in a skin-derived cell model. |
Regulatory reference: | OECD 129: on using cytotoxicity tests to estimate starting doses for acute oral systemic toxicity tests; EURL-ECVAM 2011: follow-up study on the predictive capacity of the 3T3 Neutral Red Uptake cytotoxicity assay to correctly identify substances not classified for acute oral toxicity under the EU CLP system. |
Claim: | Biocompatible and Non citotoxic |
Application field: | Chemical substances or mixtures. |
Description of the test: | The in vitro LD50 prediction test is designed to test chemicals or mixtures by evaluating their cytotoxic effect on an in vitro model of BALB/c 3T3 murine fibroblasts. |
Regulatory reference: | Proprietary method |
Claim: | Depigmenting action |
Application field: | Active ingredients, mixtures or finished products. |
Description of the test: | Evaluation of the efficacy of a sample in inhibiting the activity of the tyrosinase enzyme in a controlled in vitro direct reaction. |
Regulatory reference: | Proprietary method |
Claim: | Hair loss protection and Strenghtener |
Application field: | Active ingredients and finished products. |
Description of the test: | Quantitative evaluation of the effects of a finished product or active ingredient on cell proliferation and protein synthesis through MTT test and total protein quantification after different periods of exposure. |
Regulatory reference: | OECD n° 492 |
Claim: | Non-irritant for the eyes |
Application field: | Chemical substances or mixtures. |
Description of the test: | Identification of chemical substances or mixtures that do not require classification as (severe) irritants through evaluation of their cytotoxic effects on an in vitro reconstructed corneal epithelium model. NOTE: if the sample qualifies as irritant, further tests are required in order to determine the degree of irritant action. |
Regulatory reference: | Guidelines to Commission Regulation (EU) No 655/2013 |
Claim: | Ophthalmologically tested |
Application field: | Eye contour cosmetics or products that require to confirm the compatibility with the periocular mucosa in normal usage conditions. |
Description of the test: | An ophthalmology specialist will inspect the target area, and especially the eyelids, before the application using a fissure lamp, in order to confirm the absence of swelling, hyperemia, dryness or desquamation. The inspection also rules off alterations of the eyelashes. In the eye itself, the integrity of the cornea and conjunctiva, the absence of hyperemia, and signs of conjunctival or tarsal irritation are verified. Finally, the absence of qualitative and quantitative alterations in the tear film. The specialist then evaluates and scores erythemal response, tearing, edema and dryness after the product has been used for 30 minutes, 2 days and 30 days to quantify possible alterations induced by the sample. The volunteers also provide subjective evaluations of itching, burning, stinging or foreign body response feeling, and/or sight clouding, at the same endpoints, using the VAS (Visual Analogical Scale) score system. |