Hyaluronic acid synthesis test

Regulatory reference: Proprietary method.
Claim: Anti-aging and Reduces signs of aging
Application field: Raw materials and finished products.
Description of the test: In vitro evaluation of the synthesis of hyaluronic acid up to 48h following exposure to the sample, in several concentrations, on skin and dermal papillae fibroblasts.
Posted in Anti-aging, Anti age, Reduces signs of aging, Claim for cosmetics

Evaluation of skin smothness, elasticity and compactness

Regulatory reference: Guidelines to Commission Regulation (EU) No 655/2013
Claim: Anti-aging, Reduces signs of aging and Illuminating
Application field: Visage products for mature skin.
Description of the test: Instrumental evaluation of R0, R2 and R9 parameters.
Instrument: MPA580 Cutometer® for the determination of skin viscoelasticity.
Manufacturer: Courage-Khazaka GmbH (Germany).
The L* parameter is also quantified for skin brightness. The effect is quantified through reflection colorimetry using a CL-200 Chromameter Measurements of parameters L* and b* are performed before and after the application of the product.
Posted in Anti-aging, Anti age, Illuminating, Reduces signs of aging, Claim for cosmetics

Quantification of the increase in proliferation and activation of protein synthesis on skin cells

Regulatory reference: Proprietary method
Claim: Anti-aging and Redensifies and tones the epidermis
Application field: Finished products and active ingredients.
Description of the test: Quantitative evaluation of the efficacy of a product or active ingredient in increasing cell proliferation and protein synthesis. MTT test and protein quantification at different time intervals. The test can be performed on human keratinocytes or fibroblasts.
Posted in Anti-aging, Anti age, Redensifies and tones the epidermis, Skin elasticity and toning, Claim for cosmetics