”/>

Claim for cosmetics

Evaluation of the efficacy of a product in increasing pigmentation and stimulating melatonin production with and without sunlight exposure

Regulatory reference: Guidelines to Commission Regulation (EU) No 655/2013
Claim: Tanner
Application field: Visage and body cosmetic products.
Description of the test: A first set of baseline measurements will be performed using a Minolta CR200 Colorimeter in order to identify the phototype of the target skin region and its correlated MEDu. The measurement will be repeated at T=0 and T=X days in order to evaluate changes in skin pigmentation.
All measurements will be taken in the same target region. After the treatment, treated and untreated areas will be exposed to 3 irradiation cycles across 5 days using a tanning lamp, in order to quantify the increase in pigmentation. Measurements will be performed using a Colorimeter.
High resolution imaging will be performed using a macro objective with coaxial circular flash, after each cycle of simulated sunlight exposure.
Posted in Tanner, Blemish reduction, depigmenting, tanning, Claim for cosmetics

Anti-cellulite efficacy test through analysis of morphological changes

Regulatory reference: Guidelines to Commission Regulation (EU) No 655/2013
Claim: Reduces the esthetic effects of cellulite and Toning and firming action
Application field: Body cosmetic products.
Description of the test: Morphological analysis of the areas treated with the product by blue laser DermaTOP-V3 body scansion. The instrument can precisely quantify the changes in the volume of panniculus adiposus volumes such as micro and macro-nodules.
Posted in Toning and firming action, Reduces the esthetic effects of cellulite, Cellulite, Claim for cosmetics

Evaluation of skin smothness, elasticity and compactness

Regulatory reference: Guidelines to Commission Regulation (EU) No 655/2013
Claim: Anti-aging, Reduces signs of aging and Illuminating
Application field: Visage products for mature skin.
Description of the test: Instrumental evaluation of R0, R2 and R9 parameters.
Instrument: MPA580 Cutometer® for the determination of skin viscoelasticity.
Manufacturer: Courage-Khazaka GmbH (Germany).
The L* parameter is also quantified for skin brightness. The effect is quantified through reflection colorimetry using a CL-200 Chromameter Measurements of parameters L* and b* are performed before and after the application of the product.
Posted in Anti age, Anti-aging, Illuminating, Reduces signs of aging, Claim for cosmetics

Evaluation of skin compactness and elasticity

Regulatory reference: Guidelines to Commission Regulation (EU) No 655/2013
Claim: Firming efficacy, Improves skin tone and smoothness and Improves skin elasticity
Application field: Visage and body products.
Description of the test: Measurement of R0 and R2 parameters on 20 healthy volunteers. Instrument: MPA580 Cutometer® for the scientific quantification of the viscoelasticity of the skin.
Manufacturer: Courage-Khazaka GmbH (Germany).
Posted in Firming efficacy, Improves skin elasticity, Improves skin tone and smoothness, Skin elasticity and toning, Claim for cosmetics

Evaluation of the activation of the synthesis of collagen and fibronectin

Regulatory reference: Proprietary method
Claim: Activates the synthesis of collagen, elastin, fibronectin
Application field: Finished products and active ingredients.
Description of the test: Real time PCR analysis of the expression of collagen and fibronectin, compared to an untreated control, to determine the efficacy of a product or ingredient in activating the synthesis of these proteins involved in maintaining skin elasticity and prevent aging.
Posted in Anti age, Skin elasticity and toning, Claim for cosmetics, Activates the synthesis of collagen, elastin, fibronectin

Direct antioxidant efficacy assay

Regulatory reference: Proprietary method
Claim: Antioxidant
Application field: Finished products and substances.
Description of the test: Colorimetric evaluation of the antioxidant activity of the sample in solution on a controlled oxidation reaction. Results in terms of percentage of decrease of oxidation processes caused by free oxygen radicals.
Posted in Anti age, Antioxidant, Claim for cosmetics

OECD 442D in vitro sensitization test

Regulatory reference: OECD 442D
Claim: No skin sensitizing and Hypoallergenic
Application field: Substances or mixtures.
Description of the test: The ARE-Nrf2 method employs cytofluorometry to quantify the antioxidant response in a culture of human keratinocytes after exposure to the product.
Posted in Cosmetic raw materials, Hypoallergenic, No skin sensitizing, Claim for cosmetics

OECD 442E in vitro sensitization test

Regulatory reference: OECD 442E
Claim: No skin sensitizing and Hypoallergenic
Application field: Substances or mixtures.
Description of the test: H-CLAT cytometry method to evaluate possible changes in the expression of cell surface markers (CD86 e CD54) in cultures of THP-1 human monocytes to exclude the related sensitization pathway.
Posted in Cosmetic raw materials, Hypoallergenic, No skin sensitizing, Claim for cosmetics

Ames test

Regulatory reference: OECD 471
Claim: Non mutagenic
Application field: Raw ingredients for medical devices or cosmetics.
Description of the test: Analysis of genotoxicity of a substance according to OECD 471.
Posted in Cosmetic raw materials, Raw materials for medical devices, Claim for cosmetics, Claim for medical devices, Non mutagenic

DPRA: Direct Peptide Reactivity Assay test

Regulatory reference: OECD 442C
Claim: No skin sensitizing and Hypoallergenic
Application field: Substances or mixtures without metal compounds.
Description of the test: The DPRA is proposed to address the molecular initiating event of the skin sensitisation AOP (Adverse Outcome Pathway), namely protein reactivity, by quantifying the reactivity of test chemicals towards model synthetic peptides containing either lysine or cysteine. Cysteine and Lysine percent peptide depletion values are then used to categorise a substance in one of four classes of reactivity for supporting the discrimination between skin sensitisers and non-sensitisers.
Posted in Cosmetic raw materials, Hypoallergenic, No skin sensitizing, Claim for cosmetics